Maria N. Pavlova, Ph.D, RLATG
Seattle, WA 98105
[Send email to request phone number] 85-6790
SUMMARY
Broad expertise in developmental and molecular biology, genetics, laboratory
animal science. Detail-oriented, team player, solid track record of delivered
results.
QUALIFICATIONS
-Ph.D. in Molecular Biology, M.S. in Genetics;
-RLATG certified;
-8 years in biopharmaceutical industry;
-5 years in group management in pipeline production and research operations;
-Effective communication with variety of parties within and outside the
organization (upstream and downstream pipeline groups, management, CRO,
collaborators);
-Management and execution of several unrelated projects at the same time,
-ability to adjust projects quickly in response to changing priorities;
-Expertise in QA including biosecurity.
CAREER PROGRESSION
Primal, Inc., Nura, Inc., Omeros Corp., Seattle, WA
March 2001 - February 2009
(Primal transformed into Nura in August 2003,
Nura acquired by Omeros in August 2006)
Senior Scientist August 2006 - February 2009
Senior Scientist, Group Leader March 2003 - August 2006
Scientist, Group Leader August 2001 - March 2003
Scientist March 2001 - August 2001
-Managed mouse biology group: functions included high throughput production of
knock out (KO) mice, development and maintenance of mouse colonies, breeding
genetically modified mice (GEMs) for phenotyping and target validation:
-Built highly efficient team: in 1.5 years, 160 ES cell clones were injected, 70
KO strains produced;
-Developed and implemented high throughput genotyping protocol as part of KO
production, which increased productivity in about five times and dramatically
reduced costs;
-Produced KO/WT mice for testing of over 30 GPCR strains, which required 200
mice per strain;
-Implemented close and frequent communication with team members and downstream
behavior and physiology groups:
Daily updated team members on status of project which allowed quick schedule
adjustments and increased productivity of the group;
Made detailed production schedule for each mouse strain, asked for input from
downstream group leaders and regularly updated them on our group progress with
production;
-Served as scientific expert in genetically modified mice: genetics, GEM
generation and breeding, colony management, outsourcing;
-For two years, was first line manager of SPF animal facility which included
four companies subleasing space and services:
Developed and implemented standard operating procedures;
Executed vivarium QA programs including biosecurity;
Quarantined and re-derived mouse strains by embryo transfer and
cross-fostering;
Shipped GEMs to outside facilities within USA and abroad;
-IACUC member for over 7 years;
-Performed all functions of personnel management: selection, training,
development, assignment of duties, monitoring, performance review, promotions/lay
offs, team building.
-Combined management of groups and production/operations with R
Analyzed expression of target genes by RT-PCR and Northern;
Tested mutant mice and drug candidates in behavioral assays as part of target
validation and preclinical development;
Performed pharmacokinetic testing of drug candidates in mice and rats;
As member of GPCR database team, searched literature and identified 50 target
genes.
-Effectively interacted with diverse parties within and outside the organization
(subordinates, peers, top management, subleasing companies, CRO, vendors, service
maintenance personnel, collaborators);
-Consistently met timelines and exceeded business goals, took on additional
projects and tasks and successfully accomplished them;
-Co-authored six articles with results of research at Primal and Nura.
Postdoctoral Associate Researcher January 2000 - March 2001
Institute of Molecular Biology
Medizinische Hochschule Hannover
Hanover, Germany
(Laboratory moved from USA to Germany)
-Continued projects started at the Jackson Laboratory;
-Co-authored three articles; outcomes of research created foundation for
-further studies and publications on tc mutation (Not gene).
Postdoctoral Associate Researcher June 1996 - December 1999 Jackson Laboratory
Bar Harbor, ME
-Mammalian developmental biology and genetics: positional cloning and cell
biological analysis of three genes involved in development of mouse notochord
(Sd, tc, Pt);
-Performed fine genetic mapping of tc and Pt mutations;
-Established physical maps of chromosomal regions containing Sd and tc
mutations, sequenced BAC contigs (in collaboration) and, using bioinformatics
tools, compiled list of candidate genes;
-By chimera analysis, demonstrated that the tc mutation acts non-cell
autonomously;
-Using immunohistochemistry, showed reduction of the mitotic index in the
notochord of Pt mutants;
-Trained and supervised research assistants.
AREAS OF EXPERTISE
DEVELOPMENTAL BIOLOGY
Production of knock out and transgenic mice Isolation and culture of mouse
embryos - oocytes, morulae, blastocysts Morulae aggregation Chimera
analysis Surgeries (vasectomy, embryo transfers into uteri and oviducts)
Mouse strain re-derivation by embryo transfer, cesarian section, cross fostering
Analysis of gene expression by in situ hybridization
CELL CULTURE
ES cell culture Tissue culture of human cells (HEK 293 etc.) Isolation and
culture of mouse embryonic fibroblasts Transfection Selection of stable
clones
GENETICS
Genetic manipulations, development and maintenance of mutant mouse strains
Speed congenics Fine genetic mapping of mouse mutations using backcrosses and
polymorphic PCR markers Drosophila genetics techniques including cytogenetics
MOLECULAR BIOLOGY
Molecular Cloning, DNA modifying enzymes/techniques Sequencing and sequence
analysis Site directed mutagenesis Sterile work, bacterial transformations,
bacteriophage Lambda, selection and screening (PCR, hybridization) DNA
isolation (bacterial plasmid DNA, bacteriophage l, Drosophila, whole mouse, mouse
tissues, ES cells) High molecular weight DNA isolation for PFGE, in agarose
blocks (mouse tissues, ES cells, yeast) Screening of genomic libraries in BAC,
YAC, P1, bacteriophage l Pulse Field electrophoresis Establishing physical
maps of chromosomal regions Positional cloning Genotyping by PCR and
Southern Multiple programs and databases for sequence analysis, homology
searches, molecular cloning (Sequencher, dsGene, BLAST, UniGene, etc) RNA
isolation Northern PCR, including RT-PCR Cloning full length cDNA by
using EST, screening cDNA libraries, RACE Generation of radioactive and
fluorescent hybridization probes Whole mount in situ hybridization
Radioactive isotopes: 32P, 30P, S, 3H
HISTOLOGY
Paraffin and frozen sections Staining and processing sections Preparation
of Drosophila polytene chromosomes In situ hybridization (mouse embryos,
Drosophila polytene chromosomes) Immunohistochemistry Mouse skeletal
preparations
PHARMACOKINETICS
IP and oral drug administration Blood collection from catheter Terminal
blood collection Plasma preparation Brain isolation Bioavailability
Brain to plasma ratio
BEHAVIORAL BIOLOGY
Conditional Avoidance Response Open Field Activity Hot Plate Tail
Suspension Test Prepulse Inhibition Conditional Fear Novel Object
Recognition
LABORATORY ANIMAL SCIENCE
Mouse and rat husbandry Management of high throughput breeding of mutant and
inbred mouse strains Mouse strain re-derivation by embryo transfer and
caesarian section Cross fostering Mouse and rat diets Individually
ventilated and static cages Procurement and receiving animals Shipping
animals to outside animal facilities Anesthesia by IP injection and inhalation
Animal identification by ear tags or ear notches Biopsies (tail clipping,
ear notches) Repeated and terminal blood collection Plasma and serum
preparation Serology Blood chemistry Urine and feces collection
Urinalysis Anal tape and mites test Surgeries (vasectomy, embryo transfers
into oviducts and uteri, subcutaneous implantation of pumps for drug delivery)
IP, IV, and oral drug administration Dissections and tissue collection
Necropsy
MANAGEMENT OF ANIMAL FACILITIES
Design of animal facilities Budget planning and operational costs
Inventories Quality Assurance Equipment monitoring and maintenance HVAC
monitoring Microbial monitoring Animal health monitoring Quarantine
procedures Cage washing facilities Writing and implementation of standard
operating procedures Assistance to researchers Close interaction with
subleasing companies, CRO, vendors, service maintenance personnel, collaborators
Legislative regulations OSHA, PHS, OLAW IACUC
PERSONNEL MANAGEMENT
Selection, training, development Performance review Promotions Lay offs
Team building Leadership Increasing productivity by implementing
cooperation, flexibility, motivation Cross-training of team members
Planning Organizing Group meetings Scheduling workload Monitoring
EDUCATION
-PhD in Molecular Biology: Moscow State University, Russia.
-MS in Genetics (Departmental High Honors): Moscow State University, Russia.
CONTINUING EDUCATION/CERTIFICATIONS
-Completed two out of four exams for CMAR certification (Certified Manager of
Animal Resources, 2008), studying for the remaining two.
-RLATG certification, 2007.
-Short Course on the Pathobiology of the Modern Laboratory Mouse: Jackson
Laboratory, 2002.
-From the Laboratory to Leadership: Developing Scientific Managers: by The
Leadership Edge, 2001.
PROFESSIONAL AFFILIATIONS
-AALAS member.
SELECTED PUBLICATIONS
Zeng H, Horie K, Madisen L, Pavlova MN, Gragerova G, Rohde AD, Schimpf BA, Liang |
